Healthy Cell Proliferation

Cell proliferation assays are a cornerstone of stem cell and cancer cell pathway analysis and drug testing for efficacy and safety.

Healthy cell proliferation.

Cell counting using viability dyes such as trypan blue or calcein am can provide both the rate of proliferation as well as the percentage of viable cells. Healthy cells actively proliferate whereas growth arrested senescent and dead or dying cells do not. Cell health below is a table to aid in reagent choice for cell health and proliferation labeling applications. Cell proliferation is the biological process of cells increasing in number over time through cell division mitosis in eukaryotic cells.

3d cell cultures more closely mimic in vivo conditions but can present challenges for experimental design. Sex cells reproduce through a process called meiosis. Cells in culture are only a model system and are different than cells in their normal in vivo environment. The proliferation and self renewal of these cells depend on growth factors.

Healthy human cells usually produce arginine via arginosuccinase synthase but in tumor cells due to the lack of these enzymes it becomes auxotrophic for arginine to survive and proliferate. In addition to proliferation or cytotoxicity apoptosis necrosis and cytostatic responses can all be evaluated using cell health assays. Assays for cell proliferation may monitor the number of cells over time the number of cellular divisions metabolic activity or dna synthesis. Cell reproduction is needed to replenish the cell population that ages or becomes damaged or destroyed.

Cells communicate with other cells through chemical signals. Thus arginine offers a potential targeting therapy by limiting its level in tumor cells. Cell proliferation refers to an increase in cell number due to cell division cytokinesis which occurs as the final step of the cell cycle. Normal cells reproduce properly.

Proliferation is an essential mechanism for normal tissue development regeneration and renewal. These signals help normal cells to know when to reproduce and when to stop reproducing. Except for sex cells all cells of the body reproduce by mitosis. Cell type suitability indicates whether this reagent labels live cells exclusively or whether it can also label the dead cells in a live cell culture or cells that have been previously fixed with buffers like paraformaldehyde or methanol.

Viability is a measure of the number of living cells in a population whereas proliferation is a measure of cell division. It should be noted that not all viable cells divide. Aberrations in cell proliferation however can give rise to malignant transformation and cancer pathology.